Canine Ehrlichia Lyme Anaplasma Heartworm Rapid Test Kit is a side-flow immunochromatographic method It is used for the qualitative detection of EHR, LYM and ANA antibodies, CHW antigen and whole blood samples in dog serum and plasma. Determination time: 5-10 minutes Assay Time: 5-10 minutes

PRINCIPLE

4DX Test Dogs Ehrlichia Lyme Anaplasma Heartworm Rapid Test Kit is based on sandwich side flow immunochromatography. The test card has a test window for observing assay runs and reading results. Before running the analysis, the test window has an invisible T (test) area and a C (control) area. As the treated sample is applied to each sample well on the device, the liquid flows across the surface of the test strip and reacts with pre-coated recombinant antigens ehrlichia, Borrelia, Apthenium, and cardiacworm antibodies, respectively . A visible T-line appears if the specimen contains antibodies to Ehrlichia, borrelia, Anaplasmosis, and Carfilaria antigens. The C line should always appear after the sample is applied, indicating that the results are valid. In this way, the device can accurately indicate the presence of Ehrlichia, borrelia, anplasma antibodies and cardiacworm antigens in the specimen

REAGENTS AND MATERIALS

- 10 test pouches, with cards and disposable droppers

-10 vials of EHR-LYM-ANA assay buffer (1000μL each)

-1 bottle of CHW assay buffer (2mL)

-1 package insert

TEST PROCEDURE

- Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.

-Take out the test card from the foil pouch and place it horizontally.

-Place 10 μL of the prepared specimen into the sample hole, matching the window CHW. Then drop 3 drops (approx. 100μL) of assay buffer CHW into the sample hole. Start the timer.